Dose-Dependent Inhibition of Saccharomyces cerevisiae Growth by Thapsigargin: Insights into Antifungal Mechanisms
DOI:
https://doi.org/10.5281/zenodo.24147950Keywords:
Thapsigargin, Thapsia garganica, Saccharomyces cerevisiae, , Antifungal efficacy, Dose-response.Abstract
This study explores the inhibitory effects of thapsigargin on the growth of Saccharomyces cerevisiae. Utilizing a dose-response approach, we evaluated the antifungal efficacy of thapsigargin at concentrations ranging from 0.1 mg/mL to 5 mg/mL, incubated over 2 and 4 days. Results demonstrated a significant dose-dependent inhibition of yeast growth, with maximum inhibition observed at 5 mg/mL after 4 days. Specifically, the percentage inhibition was calculated as 63% compared to the control groups, which included ethyl acetate and sterile water. The findings indicate that thapsigargin exerts potent antifungal effects on S. cerevisiae, with potential implications for food safety and fermentation processes. This research fills existing gaps in the literature regarding the effects of plant-derived compounds on beneficial yeast species, contributing valuable insights into natural antifungal strategies. The results underscore the significance of Thapsia garganica as a viable candidate for further exploration in antifungal applications, particularly in the context of food preservation and biotechnological processes.
References
1. Abderrahim, O., Martin, G. J., & Abdelaziz, A. (2013). Comparative studies of the phytochemistry and fruit anatomy of Thapsia garganica and Thapsia transtagana, Apiaceae (Umbelliferae). Journal of Medicinal Plants Research, 7(31), 2165–2169. https://doi.org/10.5897/JMPR11.1597
2. Alilou, H., & Akssira, M. (2021). Chemical composition, antibacterial, antioxidant, and insecticidal activities of Moroccan Thapsia transtagana essential oil. Saudi Journal of Biological Sciences, 28(12), 6756–6764. https://doi.org/10.1016/j.sjbs.2021.07.052
3. Andersen, T. B., López, C. Q., Manczak, T., Martinez, K., & Simonsen, H. T. (2015). Thapsigargin: From Thapsia L. to Mipsagargin. Molecules, 20(4), 6113–6127. https://doi.org/10.3390/molecules20046113
4. Arevalo-Villena, M., Briones-Perez, A., Corbo, M. R., Sinigaglia, M., & Bevilacqua, A. (2017). Biotechnological application of yeasts in food science: Starter cultures, probiotics, and enzyme production. Journal of Applied Microbiology, 123(6), 1360-1372. https://doi.org/10.1111/jam.13548
5. Bokulich, N. A., Ohta, M., Richardson, P. M., & Mills, D. A. (2013). Monitoring seasonal changes in winery-resident microbiota. PLOS ONE, 8(6), e66437. https://doi.org/10.1371/journal.pone.0066437
6. Christensen, S. B., Norup, E., & Rasmussen, U. (1984). Chemistry and structure-activity relationship of the histamine secretagogue and Thapsigargin and related compounds. Journal of Medicinal Chemistry, 27(4), 455–460.
7. Goldstein, A. L., & McCusker, J. H. (2001). Development of Saccharomyces cerevisiae as a model pathogen: A system for the genetic identification of gene products required for survival in the mammalian host environment. Genetics, 159(2), 499-513. https://doi.org/10.1093/genetics/159.2.499
8. Li, Q., Wang, Z., Xie, Y., & Hu, H. (2020). Antitumor activity and mechanism of costunolide and dehydrocostus lactone: Two natural sesquiterpene lactones from the Asteraceae family. Biomedicine & Pharmacotherapy, 125, 109955. https://doi.org/10.1016/j.biopha.2020.109955
9. Parapouli, M., Vasileiadis, A., Afendra, A. S., & Hatziloukas, E. (2020). Saccharomyces cerevisiae and its industrial applications. AIMS Microbiology, 6(1), 1-31. https://doi.org/10.3934/microbiol.2020001
10. Schindler, D. (2020). Genetic engineering and synthetic genomics in yeast to understand life and boost biotechnology. Bioengineering, 7(4), 137. https://doi.org/10.3390/bioengineering7040137
11. Smitt, U. W., Jäger, A. K., Adsersen, A., & Gudiksen, L. (1995). Comparative studies in phytochemistry and fruit anatomy of Thapsia garganica and Thapsia transtagana, Apiaceae (Umbelliferae). Botanical Journal of the Linnean Society, 117(3), 281–292. https://doi.org/10.1006/bojl.1995.0021
12. Vanderwaeren, L., Dok, R., Voordeckers, K., Nuyts, S., & Verstrepen, K. J. (2022). Saccharomyces cerevisiae as a model system for eukaryotic cell biology, from cell cycle control to DNA damage response. International Journal of Molecular Sciences, 23(19), 11665. https://doi.org/10.3390/ijms231911665
