Dose-Dependent Inhibition of Saccharomyces cerevisiae Growth by Thapsigargin: Insights into Antifungal Mechanisms

Abstract

This study explores the inhibitory effects of thapsigargin on the growth of Saccharomyces cerevisiae. Utilizing a dose-response approach, we evaluated the antifungal efficacy of thapsigargin at concentrations ranging from 0.1 mg/mL to 5 mg/mL, incubated over 2 and 4 days. Results demonstrated a significant dose-dependent inhibition of yeast growth, with maximum inhibition observed at 5 mg/mL after 4 days. Specifically, the percentage inhibition was calculated as 63% compared to the control groups, which included ethyl acetate and sterile water. The findings indicate that thapsigargin exerts potent antifungal effects on S. cerevisiae, with potential implications for food safety and fermentation processes. This research fills existing gaps in the literature regarding the effects of plant-derived compounds on beneficial yeast species, contributing valuable insights into natural antifungal strategies. The results underscore the significance of Thapsia garganica as a viable candidate for further exploration in antifungal applications, particularly in the context of food preservation and biotechnological processes.